As an important post-transcriptional modification, N6-methyladenosine (m6A) was largely discovered by high throughput sequencing recent years. m6A was identified with consensus sequence surrounding m6A site RRACH (R=G or A, H=A, C or U) and conserved in human, mouse, chimpanzee and even in plants. m6A also existed in bacterial and archaeal species. The m6A abundance was reported being correlated with conserved region of genome. m6A modification was a reversible status mediated by methyltransferases METTL3/ METTL14/ WTAP complex, demethylases FTO/ALKBH5 and recognized by m6A binding proteins YTH domain family/HNRNPA2B1, which were called writer, eraser and reader, respectively.