As an important post-transcriptional modification, N⁶-methyladenosine (m⁶A) was largely discovered by high throughput sequencing recent years. m⁶A was identified with consensus sequence surrounding m⁶A site RRACH (R=G or A, H=A, C or U) and conserved in human, mouse, chimpanzee and even in plants. m⁶A also existed in bacterial and archaeal species. The m⁶A abundance was reported being correlated with conserved region of genome. m⁶A modification was a reversible status mediated by methyltransferases METTL3/ METTL14/ WTAP complex, demethylases FTO/ALKBH5 and recognized by m⁶A binding proteins YTH domain family/HNRNPA2B1, which were called writer, eraser and reader, respectively.